Gel Filtration Media and Columns
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Filtered Search Results
Cytiva Superose™ Media Prep Grade Lab Packs
High performance gel filtration media for scaleup to preparative purifications of biomolecules
| Particle Size | 30 to 40 μm |
|---|---|
| Flow Rate | 40 cm/hr. |
| Bed Volume | ≈100 mL |
| pH Range | 3 to 12 |
| For Use With (Equipment) | AKTA™ design pump or system |
| Sample Type | Proteins, Peptides, Polynucleotides, Wide Variety of Biomolecules |
| Quantity | Each |
| For Use With (Application) | Separations of Proteins and other biomolecules according to size |
| Support Type | Highly Cross-linked Agarose |
Cytiva Sephadex™ G15 Media
Gel filtration medium for desalting and buffer exchange for small biomolecules greater than 1500 molecular weight
| Height (Metric) | 5 to 15cm |
|---|---|
| Content And Storage | 4° to 30°C |
| Format | Solid Spherical Bead |
| Product Type | Sephadex Ion Exchange Media |
| For Use With (Equipment) | Column or Batch Techniques |
| For Use With (Application) | Separating and purifying Proteins, Polypeptides, Nucleic Acids and other charged biomolecules by using column or batch techniques |
| Support Type | Cross-linked Dextran |
| Height (English) | 2 to 5.9 in. |
Cytiva Sephacryl™ S-300 High Resolution Media
Offer good recovery and low nonspecific adsorption. Hydrophilic, rigid allyl dextran/bisacrylamide matrix for fast and reproducible purification of proteins, polysaccharides and other macromolecules. Enable gel filtration at laboratory and industrial scale, and may be purchased in pre-packed columns.
| Particle Size | 25 to 75 μm |
|---|---|
| Height (Metric) | 60cm |
| Content And Storage | 4°C to 30°C, 20% Ethanol |
| pH Range | 3 to 11 |
| Product Type | Sephacryl HR Column |
| For Use With (Equipment) | Low to medium pressure AKTA™ systems |
| Sample Type | Peptides and small proteins |
| Quantity | Each |
| Material | Polypropylene |
| For Use With (Application) | Separation of biomolecules with maximum recovery and minimum nonspecific adsorption with long term chemical and physical stability resulting from the hydrophilic, rigid Allyl Dextran/Bisacrylamide matrix |
| Support Type | Allyl Dextran and N, N'-methylenebisacrylamide |
| Max. Pressure | 1.5 bar [0.15 MPa] (22 psi) |
| Height (English) | 23.62 in. |
Cytiva Sephadex™ G-100 Media
Commonly used for desalting and buffer exchange of very large biomolecules
| Content And Storage | 4° to 30°C |
|---|---|
| Bed Volume | 15 to 20mL/g in Distilled Water |
| Format | Solid Bead |
| pH Range | 2 to 10 |
| Product Type | Sephadex G-100 Media |
| Sample Type | Large biomolecules |
| Quantity | 100 g |
| For Use With (Application) | Useful for desalting and Buffer exchange of very large biomolecules |
| Support Type | Cross-linked Dextran |
| Max. Pressure | 0.096 bar |
Cytiva Sephacryl™ S-500 High Resolution Media
Hydrophilic, rigid allyl dextran/bisacrylamide matrix for fast and reproducible purification of proteins, polysaccharides and other macromolecules
| Height (Metric) | 15cm |
|---|---|
| Content And Storage | 4° to 30°C |
| Bed Volume | 19 to 230L |
| pH Range | 3 to 11 |
| Diameter (Metric) Inner | 40 to 140cm |
| For Use With (Application) | Allow fast and reproducible purification of proteins, polysaccharides and other macromolecules by gel filtration at laborato ry and industrial scale |
| Max. Pressure | 100kPa |
| Particle Size | 25 to 75 μm |
| Flow Rate | >125 cm/hr. |
| Format | Solid Spherical Bead |
| Product Type | Sephacryl HR Media |
| Sample Type | Polysaccharides, macromolecules and small plasmids |
| Support Type | Allyl Dextran and N, N'-methylenebisacrylamide |
| Height (English) | 5.9 in. |
Cytiva Superdex™ 75 Prep Grade
Offers high resolution fractionation for separation, polishing and product formulation of recombinant proteins
Cytiva IgG Sepharose™ 6 Fast Flow Medium
Use for rapid purification of protein A and protein A fusion conjugates. IgG Sepharose™ 6 Fast Flow Medium achieves the highest flowrates.
Cytiva HiTrap™ Blue HP Columns
For purifying albumin, enzymes including NAD+ and NADP+, coagulation factors, interferons and related proteins
| Particle Size | 34 μm |
|---|---|
| Height (Metric) | 2.5cm |
| Content And Storage | 4° to 30°C |
| pH Range | 4 to 12 |
| Product Type | Blue HP Columns |
| For Use With (Equipment) | Syringe, peristaltic pump or liquid chromato graphy system such as AKTA™ design or FPLC system |
| Sample Type | Albumin, Interferon, Nucleotide-dependent Enzymes, a2-macroglobulin, Coagulation Factors and Related Proteins |
| Material | Polypropylene |
| For Use With (Application) | Purifying wide range of biomolecules, including albumin, interferon, a broad range of nucleotide-dependent Enzymes, alpha2-macroglobulin, coagulation facto rs and related Proteins |
| Support Type | 6% Highly Cross-linked Agarose |
| Max. Pressure | 42psi |
| Height (English) | 0.98 in. |
Cytiva Sephadex™ G-25 DNA Grade SF Cleanup Gel
Tested to ensure reproducibly high recovery of DNA (10 bases in length) from small molecules by gel filtration
| Particle Size | 100 to 300 μm in wet form, 50 to 150 μm in dry form |
|---|---|
| Height (Metric) | 10cm |
| Bed Volume | 9 to 11mg/g |
| Format | Solid Bead |
| pH Range | 2 to 10 |
| Product Type | Sephadex G50 Media |
| Sample Type | DNA |
| Diameter (Metric) Inner | 5cm |
| Support Type | Cross-linked Dextran |
| Height (English) | 3.93 in. |
Cytiva Superdex 30 PG size exclusion chromatography media prepacked into HiLoad 26/600 column
Superdex 30 prep grade (PG) size exclusion chromatography (gel filtration) media prepacked into a HiLoad 26/600 column is optimized for the separation of proteins and peptides, but can also be used with success to separate oligosaccharides.